- Appomattox River
- Big Tumbling Creek (Clinch Fee Fishing Area)
- Blackwater River
- Chickahominy River
- Clinch River
- Conway River
- Cowpasture River
- Dan River
- Dragon Run / Piankatank River
- Holston River – Middle Fork
- Holston River – North Fork
- Holston River – South Fork
- Jackson River
- James River – Tidal
- James River – Upper & Middle
- Mattaponi River
- Maury River
- Meherrin River
- Mossy Creek
- New River
- North Fork Moormans River
- North Fork Thorton River
- North Landing and Northwest Rivers
- North River
- Nottoway River
- Occoquan River
- Pamunkey River
- Pound River
- Powell River
- Rapidan River (Trout Section)
- Rappahannock River – Tidal
- Rappahannock River – Upper
- Rivanna River
- Shenandoah River – Main Stem
- Shenandoah River – North Fork
- Shenandoah River – South Fork
- Smith River
- South River
- St. Marys River
- Staunton River
- Whitetop Laurel