| Allegheny Mountain Dusky Salamander | Desmognathus ochrophaeus | |
| American Bullfrog | Lithobates catesbeianus | |
| Atlantic Coast Slimy Salamander | Plethodon chlorobryonis | |
| Barking Treefrog | Hyla gratiosa | |
| Big Levels Salamander | Plethodon sherando | |
| Black Mountain Salamander | Desmognathus welteri | |
| Black-Bellied Salamander | Desmognathus quadramaculatus | |
| Blue Ridge Dusky Salamander | Desmognathus orestes | |
| Blue Ridge red salamander | Pseudotriton ruber nitidus | (Plethodontidae) |
| Blue Ridge Two-Lined Salamander | Eurycea wilderae | |
| Brimley’s Chorus Frog | Pseudacris brimleyi | |
| Carpenter Frog | Lithobates virgatipes | |
| Cave Salamander | Eurycea lucifuga | |
| Coastal Plains Leopard Frog | Lithobates sphenocephalus utriculariu | |
| Common Mudpuppy | Necturus maculosus maculosus | |
| Cope’s Gray Treefrog | Hyla chrysoscelis | |
| Cow Knob Salamander | Plethodon punctatus | |
| Cumberland Plateau Salamander | Plethodon kentucki | |
| Dixie Caverns Salamander | | |
| Dwarf Waterdog | Necturus punctatus | |
| Eastern American Toad | Anaxyrus americanus americanus | |
| Eastern Cricket Frog | Acris crepitans | |
| Eastern Hellbender | Cryptobranchus alleganiensis alleganiensis | (Cryptobranchidae) |
| Eastern Lesser Siren | Siren intermedia intermedia | (Sirenidae) |
| Eastern Long-Tailed Salamander | Eurycea longicauda longicauda | |
| Eastern Narrow-Mouthed Toad | Gastrophryne carolinensis | |
| Eastern Red-Backed Salamander | Plethodon cinereus | |
| Eastern Spadefoot | Scaphiopus holbrookii | |
| Eastern Tiger Salamander | Ambystoma tigrinum | |
| Flat-Headed Salamander | Desmognathus planiceps | |
| Four-Toed Salamander | Hemidactylium scutatum | |
| Fowler’s Toad | Anaxyrus fowleri | |
| Gray Treefrog | Hyla versicolor | |
| Greater Siren | Siren lacertina | |
| Green Frog | Lithobates clamitans | |
| Green Salamander | Aneides aeneus | |
| Green Treefrog | Hyla cinerea | |
| Holbrook’s Southern Dusky Salamander | Desmognathus auriculatus | |
| Jefferson’s Salamander | Ambystoma jeffersonianum | |
| Kentucky spring salamander | Gyrinophilus porphyriticus duryi | (Plethodontidae) |
| Little Grass Frog | Pseudacris ocularis | |
| Mabee’s Salamander | Amybstoma mabeei | |
| Many-Lined Salamander | Stereochilus marginatus | |
| Marbled Salamander | Ambystoma opacum | |
| Mid-Atlantic Coast Leopard Frog | Lithobates kauffeldi | |
| midland mud salamander | Pseudotriton montanus diastictus | (Plethodontidae) |
| Mole Salamander | Ambystoma talpoideum | |
| Mountain Chorus Frog | Pseudacris brachyphona | |
| Mud Salamander | Pseudotriton montanus | |
| New Jersey Chorus Frog | Pseudacris kalmi | |
| Northern Cricket Frog | Acris crepitans | (Hylidae) |
| Northern Dusky Salamander | Desmognathus fuscus | |
| Northern Gray-Cheeked Salamander | Plethodon montanus | |
| Northern Pygmy Salamander | Desmognathus organi | |
| Northern Slimy Salamander | Plethodon glutinosus | |
| northern spring salamander | Gyrinophilus porphyriticus porphyriticus | (Plethodontidae) |
| Northern Two-Lined Salamander | Eurycea bislineata | |
| Oak Toad | Anaxyrus quercicus | (Bufonidae) |
| Peaks of Otter Salamander | Plethodon hubrichti | |
| Pickerel Frog | Lithobates palustris | |
| Pine Woods Treefrog | Hyla femoralis | |
| Red Salamander | Pseudotriton ruber | |
| Red-Spotted Newt | Notophthalmus viridescens | |
| Seal Salamander | Desmognathus monticola | |
| Shenandoah Mountain Salamander | Plethodon virginia | |
| Shenandoah Salamander | Plethodon shenandoah | |
| Shovel-Nosed Salamander | Desmognathus marmoratus | |
| Southern Chorus Frog | Pseudacris nigrita | |
| Southern Cricket Frog | Acris gryllus | |
| Southern Ravine Salamander | Plethodon richmondi | |
| Southern Toad | Anaxyrus terrestris | |
| Southern Two-Lined Salamander | Eurycea cirrigera | |
| Southern Zigzag Salamander | Plethodon ventralis | |
| Spotted Salamander | Ambystoma maculatum | Mole salamander (Ambystomatidae) |
| Spring Peeper | Pseudacris crucifer | |
| Spring Salamander | Gyrinophilus porphyriticus danielsi | |
| Squirrel Treefrog | Hyla squirella | |
| Three-Lined Salamander | Eurycea guttolineata | (Plethodontidae) |
| Two-Toed Amphiuma | Amphiuma means | |
| Upland Chorus Frog | Pseudacris feriarum | |
| Valley And Ridge Salamander | Plethodon hoffmani | |
| Wehrle’s Salamander | Plethodon wehrlei | |
| Weller’s Salamander | Plethodon welleri | |
| White-Spotted Slimy Salamander | Plethodon cylindraceus | |
| Wood Frog | Lithobates sylvaticus | |
| Yonahlossee Salamander | Plethodon yonahlossee | |